PUBLICATIONS

1. Dalakouras A, Vlachostergios D. 2021. Epigenetic approaches for crop breeding: current status and perspectives. J Exp Bot. doi: 10.1093/jxb/erab227 (IF 5,9)
2. Dalakouras A, Ganopoulos I. 2021. Induction of Promoter DNA Methylation Upon High-Pressure Spraying of Double-Stranded RNA in Plants. Agronomy 11, 789. doi.org/10.3390/agronomy11040789 (IF 2,6)
3. Wassenegger M, Dalakouras A. 2021. Viroids as a Tool to Study RNA-Directed DNA Methylation in Plants. Cells 10, 1187. doi.org/10.3390/cells10051187 (IF 4,4)
4. Dalakouras, A. and Papadopoulou, K. (2020) Epigenetic modifications: an unexplored facet of exogenous RNA application in plants. Plants 9(6):673
5. Dalakouras, A., Wassenegger, M., Dadami, E., Ganopoulos, I., Pappas, M. and Papadopoulou K. (2020) Genetically modified organism free RNA interference: Exogenous application of RNA molecules in plants. Plant Physiol. 182(1):38-50
6. Xanthopoulou, A., Montero-Pau J., Mellidou, I., Kissoudis, C., Blanca, J., Picó, B., Tsaballa, A., Tsaliki, E., Dalakouras, A., Paris, H., Ganopoulou, M., Moysiadis, T., Osathanunkul, M., Tsaftaris, A., Madesis, P., Kalivas, A. and Ganopoulos, I. (2019) Whole-genome resequencing of Cucurbita pepo morphotypes to discover genomic variants associated with morphology and horticulturally valuable traits. Hortic Res. 6:94
7. Dalakouras, A*., Lauter, A., Bassler, A., Krczal, G. and Wassenegger, M. (2019) Transient expression of intron-containing transgenes generates aberrant pre-mRNAs that are processed to siRNAs. Planta. 249(2):457-468 *corresponding author
8. Dalakouras, A*., Jarausch, W., Buchholz, G., Bassler, A., Braun, M., Manthey, T., Krczal, G. and Wassenegger, M. (2018) Delivery of exogenous RNA molecules to woody and herbaceous plants by trunk injection and petiole absorption. Front Plant Sci. 9:1253. *corresponding author
9. Dadami, E., Dalakouras, A. and Wassenegger, M. (2017) Viroids and RNA silencing. Viroids and Satellites, pp.115-124.
10. Dalakouras, A., Wassenegger, M., McMillan, J., Cardoza, V., Maegele, I., Dadami, E., Runne, M., Krczal, G. and Wassenegger, M. (2016) Induction of silencing in plants by high-pressure spraying of in vitro-synthesized small RNAs. Front Plant Sci. 7.
11. Dalakouras, A., Dadami, E., Wassenegger, M., Krczal, G. and Wassenegger, M. (2016) RNA-directed DNA methylation efficiency depends on trigger and target sequence identity. Plant J. 87(2), 202-214.
12. Dalakouras, A., Dadami, E. and Wassenegger, M. (2015) Engineering viroid resistance. Viruses, 7, 634-646.
13. Dalakouras, A., Dadami, E., Bassler, A., Zwiebel, M., Krczal, G. and Wassenegger, M. (2015) Replicating Potato spindle tuber viroid mediates de novo methylation of an intronic viroid sequence but no cleavage of the corresponding pre-mRNA. RNA boil. 12, 268-275.
14. Saeed, M., Briddon, R.W., Dalakouras, A., Krczal, G. and Wassenegger, M. (2015) Functional Analysis of Cotton Leaf Curl Kokhran Virus/Cotton Leaf Curl Multan Betasatellite RNA Silencing Suppressors. Biology. 4, 697-714
15. Dadami, E., Dalakouras, A*., Zwiebel, M., Krczal, G. and Wassenegger, M. (2014) An endogene-resembling transgene is resistant to DNA methylation and systemic silencing. RNA Biol. 11, 934-941. * equal contribution
16. Dadami, E., Moser, M., Zwiebel, M., Krczal, G., Wassenegger, M. and Dalakouras, A.* (2013) An endogene-resembling transgene delays the onset of silencing and limits siRNA accumulation. FEBS Lett. 18, 706-710. *corresponding author
17. Dalakouras, A. and Wassenegger, M. (2013) Revisiting RNA-directed DNA methylation. RNA Biol. 10, 453-455.
18. Dalakouras, A*., Dadami, E. and Wassenegger, M. (2013) Viroid-induced DNA methylation in plants. BioMol Concepts, 4, 557-565. *corresponding author
19. Dalakouras, A., Dadami, E., Zwiebel, M., Krczal, G. and Wassenegger, M. (2012) Transgenerational maintenance of transgene body CG but not CHG and CHH methylation. Epigenetics, 7, 1071-1078.
20. Dalakouras, A., Moser, M., Boonrod, K., Krczal, G. and Wassenegger, M. (2011) Diverse spontaneous silencing of a transgene among two Nicotiana species. Planta, 234, 699-707.
21. Dalakouras, A., Tzanopoulou, M., Tsagris, M., Wassenegger, M. and Kalantidis, K. (2011) Hairpin transcription does not necessarily lead to efficient triggering of the RNAi pathway. Transgenic Res. 20, 293-304.
22. Dalakouras, A., Moser, M., Krczal, G. and Wassenegger, M. (2010) A chimeric satellite transgene sequence is inefficiently targeted by viroid-induced DNA methylation in tobacco. Plant Mol Biol. 73, 439-447.
23. Dalakouras, A., Moser, M., Zwiebel, M., Krczal, G., Hell, R. and Wassenegger, M. (2009) A hairpin RNA construct residing in an intron efficiently triggered RNA-directed DNA methylation in tobacco. Plant J. 60, 840-851.
24. Dalakouras, T., Smith, B.J., Platis, D., Cox, M.M. and Labrou, N.E. (2006) Development of recombinant protein-based influenza vaccine. Expression and affinity purification of H1N1 influenza virus neuraminidase. J Chromatogr A. 1136, 48-56.

EUROPEAN PATENT

Wassenegger, Μ., Krczal, G. and Dalakouras, A. (2010) Method for the production of a transgene-free plant with altered methylation pattern (WO 2010/066343, PCT/EP2009/008437).